Full Length Research Paper

Identification of iridovirus based on molecular and imunohistochemistry studies on the grouper fish (Epinephelus sp.) in Lombok, Indonesia

Fadli Ma’mun¹, Kurniasih Imanudin² and Tri Untari³

Abstract

Grouper cultivation in floating net cages in Lombok is growing well in Indonesian. Problem of grouper fish production is high mortality rates caused by Iridovirus infection. Aim of study is to determine the incidence of Iridovirus infection in grouper fish based on polymerase chain reaction (PCR) and immunohistochemistry test in Lombok Island. A total of 25 fish grouper with size ± 10 cm length infected naturally iridovirus was collected from floating net cages. Internal organs were fixed with 10% of phosphate-buffer formalin for immunohistochemistry examination and in absolute ethanol of solution for molecular study. The positive result of polymerase chain reaction the bright band at 570 bp. Positive results of Iridovirus showed that 9 from 10 samples of cantang grouper in East Lombok were positif iridovirus infection, and 13 from 15 samples were positive in Central Lombok. The same results were also shown using immunohistochemistry test with antibody from rabbit. The strong immunolabellings stained brown colour were obtained from the spleen and liver. This indicated that the causative agent of high mortality in grouper fish in Lombok was caused by Iridovirus.

Key words: Iridovirus, PCR, immunohistochemistry, Lombok.

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